NanoScan OP800 Piezo Objective Scanner

The OP800 Piezo Objective Scanner provides the fastest step and settle time of any objective positioner available. Incorporating capacitive feedback sensors, it also has market-leading positioning accuracy and resolution. Compatible with most microscopes and objective lenses the system has user-configurable settings optimized for different objective sizes, weights, and performance needs. The user simply selects the best setting for their application.

Suggested controller:

• NPC-D-6110

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“We have recently added the Prior NanoScan OP800 to our imaging setup. We have had success using this device. It was easy to install onto our microscope nosepiece using brass adapter rings and we have easy control using the included software. For our imaging, our objective is to capture neuronal fluorescence that is distributed over a curved surface. On its own, our objective only allows us to visualize a small portion of this surface corresponding to a narrow focal plane. The NanoScan OP800 has enabled us to double the effective measurement field. We have programmed the device to cover a distance of 300 μm in 100 μm steps during our acquisition window of 200 ms. The device can step through these planes almost instantaneously and we observe no obvious movement artifact. We find a decrease in resolution that is expected from acquiring in multiple planes. Post-experiment image processing, reveals that this diminished resolution does not interfere with our downstream analysis. Importantly, we only find that we gain additional fluorescent signal in the previously out-of-focus planes without any information loss from the original, narrower focal field. We have attached processed images from our recording sessions of equivalent neuronal fluorescence acquired with and without the NanoScan OP800 to demonstrate the difference in our data.”

Joshua J. Emrick, DDS, PhD at The University of Michigan School of Dentistry

Images are of a GCaMP6f fluorescence in the trigeminal ganglion Courtesy of Akash R. Gandhi, Elizabeth A. Ronan, PhD, and Joshua J. Emrick, DDS, PhD, Department of Biologic and Materials Sciences & Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan.